Shigemoto R, Nakaya Y, Nomura S, Ogawa-Meguro R, Ohishi H, Kaneko T, Nakanishi S, Mizuno N
Department of Morphological Brain Science, Faculty of Medicine, Kyoto University, Japan.
Neurosci Lett. 1993 Apr 30;153(2):157-60. doi: 10.1016/0304-3940(93)90311-8.
A trp E fusion protein containing a C-terminal portion of the rat substance P receptor (SPR) was expressed in bacteria and used to produce an antibody. The antibody specifically reacted with SPR expressed in a mammalian cell line and rat striatum. Light and electron microscope analyses of the rat striatum revealed intense SPR-like immunoreactivity in neuronal somata and dendrites. These immunoreactive neurons constituted approximately 3% of the total population of striatal neurons; they were putative interneurons of large and medium-sized aspiny type.
一种包含大鼠P物质受体（SPR）C末端部分的trp E融合蛋白在细菌中表达，并用于制备抗体。该抗体与在哺乳动物细胞系和大鼠纹状体中表达的SPR特异性反应。对大鼠纹状体的光镜和电镜分析显示，在神经元胞体和树突中有强烈的SPR样免疫反应性。这些免疫反应性神经元约占纹状体神经元总数的3%；它们是假定的大中型无棘型中间神经元。
