Takeuchi T, Morimoto K, Kosaka H, Shiga T
Department of Hygiene and Preventive Medicine, Osaka University School of Medicine, Japan.
Biochem Biophys Res Commun. 1993 Jul 15;194(1):57-64. doi: 10.1006/bbrc.1993.1784.
By ESR using 5,5-dimethyl-1-pyrroline-1-oxide as a spin trap, superoxide (O2-) production was proved upon stimulation of dimethyl sulfoxide-differentiated HL60 by crocidolite opsonized with fresh or refrigerated serum, as well as by phorbol myristate acetate (PMA). Crocidolite, unopsonized or opsonized with frozen-thawed or heat-inactivated serum, did not induce O2- release. Addition of iron chelators or superoxide dismutase inhibited O2- release completely. Neither undifferentiated nor PMA-differentiated HL60 released O2- upon stimulation with opsonized crocidolite.
通过以5,5 - 二甲基 - 1 - 吡咯啉 - 1 - 氧化物作为自旋捕集剂的电子自旋共振（ESR）方法，在用新鲜或冷藏血清调理的青石棉刺激二甲基亚砜分化的HL60细胞时，以及在用佛波酯（PMA）刺激时，均证实有超氧阴离子（O2-）产生。未调理的青石棉，或用冻融或热灭活血清调理的青石棉，均未诱导O2-释放。添加铁螯合剂或超氧化物歧化酶可完全抑制O2-释放。在用调理的青石棉刺激时，未分化的HL60细胞和PMA分化的HL60细胞均不释放O2-。
