Nishihara K, Fukuda T, Tsuneyoshi M, Kominami T, Maeda S, Saku M
Second Department of Pathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Cancer. 1993 Aug 1;72(3):689-96. doi: 10.1002/1097-0142(19930801)72:3<689::aid-cncr2820720311>3.0.co;2-s.
In 1989, Morohoshi et al. reported an intraductal papillary neoplasm of the pancreas (IPNP), which was a morphologically distinct, but rare tumor.
Two cases with IPNP were analyzed by immunohistochemical and DNA flow cytometric methods.
The patients included a 67-year-old man and a 71-year-old woman. Both tumors were characterized by a well-defined papillary growth in the cystically dilated main pancreatic ducts, associated with papillary and nonpapillary hyperplasia. Immunohistochemically, the tumor cells of both cases were positive for the epithelial markers (AE1/AE3 and CAM 5.2), and in one of the two cases, the tumor cells and hyperplastic cells surrounding the tumor conspicuously revealed multiple hormonal markers such as serotonin, somatostatin, glucagon, gastrin, and pancreatic polypeptide. The nuclear DNA content of the tumor cells of the first case, which showed moderate cellular atypia, was considered to be diploid, whereas that of the second case, which revealed severe atypia, was aneuploid.
These results suggested that these tumors arose from multipotential stem cells capable of epithelial and neuroendocrine differentiation, and results of the flow cytometric study was related to the degree of cellular atypia of the tumors.
1989年，诸星等报道了胰腺导管内乳头状肿瘤（IPNP），这是一种形态学上独特但罕见的肿瘤。
采用免疫组织化学和DNA流式细胞术方法分析2例IPNP病例。
患者包括一名67岁男性和一名71岁女性。两个肿瘤均表现为在囊性扩张的主胰管内有界限清楚的乳头状生长，并伴有乳头状和非乳头状增生。免疫组织化学显示，两例肿瘤细胞的上皮标志物（AE1/AE3和CAM 5.2）均呈阳性，且在其中一例中，肿瘤细胞及肿瘤周围的增生细胞显著显示出多种激素标志物，如5-羟色胺、生长抑素、胰高血糖素、胃泌素和胰多肽。第一例肿瘤细胞的核DNA含量显示中等程度的细胞异型性，被认为是二倍体，而第二例显示严重异型性，其核DNA含量为非整倍体。
这些结果提示这些肿瘤起源于能够进行上皮和神经内分泌分化的多能干细胞，并且流式细胞术研究结果与肿瘤的细胞异型性程度相关。
