Kaplan M H, Smith D I, Sundick R S
Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI 48201.
J Immunol. 1993 Jul 15;151(2):628-36.
Many genes are induced after T cell activation to make a cell competent for proliferation and ultimately, function. Many of these genes encode surface receptors for growth factors that signal a cell to proliferate. We have cloned a novel gene (clone 6H1) that codes for a member of the G protein-coupled receptor superfamily. This gene was isolated from a chicken activated T cell cDNA library by low level hybridization to mammalian IL-2 cDNA probes. The 308 amino acid open reading frame has seven hydrophobic, presumably transmembrane domains and a consensus site for interaction with G proteins. Tissue distribution studies suggest that gene expression is restricted to activated T cells. The message appears by 1 h after activation and is maintained for at least 45 h. Transcription of 6H1 is induced by a number of T cell stimuli and is inhibited by cyclosporin A, but not by cycloheximide. This is the first description of a member of this superfamily expressed specifically in activated T cells. The gene product may provide a link between T cell growth factors and G protein activation.
许多基因在T细胞活化后被诱导表达，使细胞具备增殖能力并最终发挥功能。其中许多基因编码生长因子的表面受体，这些受体向细胞发出增殖信号。我们克隆了一个新基因（克隆6H1），它编码G蛋白偶联受体超家族的一个成员。该基因是通过与哺乳动物白细胞介素-2 cDNA探针进行低水平杂交，从鸡活化T细胞cDNA文库中分离出来的。这个308个氨基酸的开放阅读框有七个疏水的、可能是跨膜的结构域，以及一个与G蛋白相互作用的共有位点。组织分布研究表明，该基因的表达仅限于活化的T细胞。活化后1小时出现该信息，并至少维持45小时。6H1的转录受到多种T细胞刺激的诱导，并且被环孢素A抑制，但不被放线菌酮抑制。这是对该超家族中一个在活化T细胞中特异性表达的成员的首次描述。该基因产物可能在T细胞生长因子和G蛋白激活之间提供联系。
