Aune T M, McGrath K M, Sarr T, Bombara M P, Kelley K A
Institute for Inflammation and Autoimmunity, Miles Research Center, West Haven, CT 06516.
J Immunol. 1993 Aug 1;151(3):1175-83.
The neurotransmitter, serotonin (5-hydroxytryptamine, 5HT), has been shown to affect function of cells of the immune system. More recently, specific 5HT receptors have been identified and partially characterized on Jurkat cells. Results presented here characterize the receptor on Jurkat cells as the 5HT1a receptor subtype and show that mitogen-activated but not resting human T cells also express the 5HT1a receptor subtype. Analysis of mRNA in Jurkat cells and activated and resting T cells by PCR or by Northern analysis revealed the presence of 5HT1a receptor. Pharmacologic analysis of this receptor demonstrated that the receptors on Jurkat cells and activated T cells are similar to each other and that they resemble the 5HT1a receptor found in the brain. Analysis of the second messenger pathways activated by the 5HT1a receptor show that ligand-binding to the Jurkat cell 5HT1a receptor results in elevation of intracellular inositol phosphates and Ca2+ and that ligand binding to the 5HT1a receptor on activated T cells modulated intracellular levels of cAMP.
神经递质5-羟色胺（5-HT）已被证明会影响免疫系统细胞的功能。最近，在Jurkat细胞上已鉴定出特定的5-HT受体并对其进行了部分表征。本文给出的结果表明，Jurkat细胞上的该受体为5-HT1a受体亚型，且有丝分裂原激活的而非静息的人T细胞也表达5-HT1a受体亚型。通过聚合酶链反应（PCR）或Northern印迹分析对Jurkat细胞以及激活的和静息的T细胞中的信使核糖核酸（mRNA）进行分析，结果显示存在5-HT1a受体。对该受体进行的药理学分析表明，Jurkat细胞和激活的T细胞上的受体彼此相似，且类似于在大脑中发现的5-HT1a受体。对由5-HT1a受体激活的第二信使途径进行的分析表明，配体与Jurkat细胞5-HT1a受体结合会导致细胞内肌醇磷酸酯和钙离子（Ca2+）水平升高，而配体与激活的T细胞上的5-HT1a受体结合则会调节细胞内环磷酸腺苷（cAMP）的水平。
