Twiss J L, Anderson L J, Horoupian D S
Department of Pathology (Neuropathology), Stanford University Medical Center, CA 94305.
Acta Neuropathol. 1993;85(6):658-62. doi: 10.1007/BF00334677.
We report of a 27-month-old boy with a recurrent infratentorial ependymoma; the initial resection was at 14 months of age. Both resection specimens were histologically similar. In addition to neoplastic ependymal cells forming perivascular pseudo-rosettes, a second population of cells with identical nuclear morphology displayed large hyaline, refractile cytoplasmic inclusion, causing these cells to superficially resemble gemistocytic astrocytes. These inclusions demonstrated strong glial fibrillary acidic protein (GFAP) immunoreactivity. Ultrastructurally, the inclusions appeared as fenestrated irregular-shaped bodies with jagged edges and were made up of electron-dense granular material. Although these inclusions superficially resembled Rosenthal fibers, immunoperoxidase stains for ubiquitin and alpha B-crystallin were negative. Immunoelectron microscopy showed that these unusual non-filamentous inclusions were diffusely GFAP positive.
我们报告了一名27个月大的患有复发性幕下室管膜瘤的男孩；首次切除手术在其14个月大时进行。两个切除标本的组织学特征相似。除了形成血管周围假菊形团的肿瘤性室管膜细胞外，另一群具有相同核形态的细胞显示出大的透明、折光性细胞质包涵体，使这些细胞表面上类似于肥胖型星形胶质细胞。这些包涵体显示出强烈的胶质纤维酸性蛋白（GFAP）免疫反应性。在超微结构上，这些包涵体表现为边缘参差不齐的有窗孔的不规则形状物体，由电子致密的颗粒物质组成。尽管这些包涵体表面上类似于罗森塔尔纤维，但泛素和αB晶状体蛋白的免疫过氧化物酶染色均为阴性。免疫电子显微镜显示这些不寻常的非丝状包涵体弥漫性GFAP阳性。
