Daniels I, Lindsay M, Porter C, Haynes A P, Fletcher J, Morgan A G
Medical Research Centre, City Hospital, Nottingham, UK.
Nephron. 1993;64(3):382-7. doi: 10.1159/000187358.
Peritoneal dialysis effluent (PDE) contains at least 2 factors capable of affecting superoxide generation by polymorphonuclear neutrophils (PMN) in response to both particulate and soluble stimuli. A low molecular weight fraction (< 1.2 kD) enhanced the response to the chemotactic peptide fMLP and to preopsonised Staphylococcus epidermidis and Candida guilliermondii. A higher molecular weight fraction (> 1.2 kD) inhibited superoxide production in response to phorbol myristate acetate (PMA). The effects of PDE were dose dependent over the range of 10-70% (v/v) and simply augmented and reduced the dose-response curve to fMLP and PMA, respectively. There was no alteration in the concentration of stimulus required to give maximal superoxide production in either case. These data suggest that factors capable of affecting superoxide production by PMN accumulate in uraemia and are removed from the circulation into dialysis fluid.
腹膜透析流出液（PDE）含有至少两种能够影响多形核中性粒细胞（PMN）在受到颗粒性和可溶性刺激时产生超氧化物的因子。一种低分子量组分（<1.2 kD）增强了对趋化肽fMLP以及对经预调理的表皮葡萄球菌和季也蒙假丝酵母的反应。一种较高分子量组分（>1.2 kD）抑制了对佛波酯肉豆蔻酸酯乙酸酯（PMA）的超氧化物产生。PDE的作用在10 - 70%（v/v）范围内呈剂量依赖性，分别简单地增强和降低了对fMLP和PMA的剂量反应曲线。在这两种情况下，产生最大超氧化物所需的刺激浓度均未改变。这些数据表明，能够影响PMN产生超氧化物的因子在尿毒症中蓄积，并从循环中被清除到透析液中。
