Osathanondh R, Tulchinsky D, Kamali H, Fencl M, Taeusch H W
J Pediatr. 1977 Apr;90(4):617-20. doi: 10.1016/s0022-3476(77)80383-1.
Dexamethasone concentration was measured in plasma and amniotic fluid by radioimmunoassay using a rabbit antiserum raised against DX-hemisuccinate-albumin. Recoveries of added tracers averaged 70% after paper chromatography. The within- and between-assay coefficients of variation averaged 10%. The lower limit of detection was 0.2 mug/dl when 0.4 ml of plasma was assayed. Ten healthy pregnant women at term had cesarean sections 8 to 11 hours following administration of 8 mg of DX orally. DX levels in maternal vein, in umbilical vein and artery, and in amniotic fluid averaged 2.2, 2.9, 2.6, and 2.5 mug/dl, respectively. Although cortisol levels were markedly suppressed, the total relative glucocorticoid activity in blood of fetuses treated with DX far exceeded that of the untreated group.
使用针对半琥珀酸地塞米松 - 白蛋白产生的兔抗血清，通过放射免疫分析法测定血浆和羊水中的地塞米松浓度。纸层析后，添加示踪剂的回收率平均为70%。测定内和测定间变异系数平均为10%。当检测0.4ml血浆时，检测下限为0.2μg/dl。10名足月健康孕妇在口服8mg地塞米松后8至11小时进行剖宫产。母体静脉、脐静脉和动脉以及羊水中的地塞米松水平分别平均为2.2、2.9、2.6和2.5μg/dl。虽然皮质醇水平受到明显抑制，但接受地塞米松治疗的胎儿血液中的总相对糖皮质激素活性远远超过未治疗组。
