Meyer M P, Carbonell R I, Mauser N A, Kanbour A I, Amortegui A J
Department of Pathology, Magee-Womens Hospital, Pittsburgh, PA 15213.
Am J Clin Pathol. 1993 Jul;100(1):12-7. doi: 10.1093/ajcp/100.1.12.
Human papillomavirus (HPV) detection in cervical swab specimens by ViraPap and in cervical biopsies by the ViraType in situ hybridization assay was correlated with concurrent cytologic and histologic findings in 149 patients. Seven percent of patients with normal samples were positive for HPV DNA, compared with 29% and 69% of patients with low- and high-grade squamous intraepithelial lesions (SILs), respectively. The performance of both assays increased the rate of HPV detection in low-grade lesions but not in high-grade cases. ViraPap was more sensitive than the ViraType in situ hybridization assay in high-grade lesions. The present investigation suggests that HPV DNA detection may be useful in separating confirmed lesions from histologically questionable biopsies.
通过ViraPap检测宫颈拭子标本中的人乳头瘤病毒（HPV），并通过ViraType原位杂交检测法检测宫颈活检组织中的HPV，将其与149例患者同时进行的细胞学和组织学检查结果进行关联分析。正常样本患者中7%的HPV DNA呈阳性，而低度和高度鳞状上皮内病变（SIL）患者中的这一比例分别为29%和69%。两种检测方法的应用提高了低度病变中HPV的检测率，但对高度病变则不然。在高度病变中，ViraPap比ViraType原位杂交检测法更敏感。本研究表明，HPV DNA检测可能有助于从组织学上有疑问的活检组织中区分出确诊的病变。
