García-Ladona F J, Claro E, García A, Picatoste F
Instituto de Biología Fundamental, Facultad de Medicina, Universidad Autónoma de Barcelona, Spain.
Neuroreport. 1993 Jun;4(6):691-4. doi: 10.1097/00001756-199306000-00022.
We have studied the effects of the unilateral electrolytic lesion of the medial forebrain bundle at the level of the lateral hypothalamus on the density and functionality of histamine H1 receptors in rat brain cortex. The treatment resulted, after two and four weeks, in an increase in the maximal phosphoinositide breakdown induced by histamine, which can be accounted for by the appearance of a higher potency component for the response. On the other hand, the density of cortical histamine H1 receptors, determined by the specific binding of [3H]mepyramine to membranes, remained unchanged two weeks after the lesion but after four weeks a small but significant increase was also found. These results suggest that the denervation hypersensitivity developed may initially be the result of a more efficient coupling of the H1 receptors to the effector system prior to the subsequent increase in receptor numbers.
我们研究了在下丘脑外侧水平对内侧前脑束进行单侧电解损伤，对大鼠大脑皮层中组胺H1受体的密度和功能的影响。在损伤后两周和四周，该处理导致组胺诱导的最大磷酸肌醇分解增加，这可由反应中出现更高效的成分来解释。另一方面，通过[3H]美吡拉敏与膜的特异性结合测定的皮层组胺H1受体密度，在损伤后两周保持不变，但在四周后也发现有小幅度但显著的增加。这些结果表明，所产生的去神经超敏反应最初可能是H1受体与效应系统更有效偶联的结果，随后才出现受体数量增加。
