促甲状腺激素释放激素和多巴胺对富集的催乳素细胞和促甲状腺激素细胞中环磷酸腺苷水平的影响。

Effect of TRH and dopamine on cyclic AMP levels in enriched mammotroph and thyrotroph cells.

作者信息

Barnes G D, Brown B L, Gard T G, Atkinson D, Ekins R P

出版信息

Mol Cell Endocrinol. 1978 Dec;12(3):273-84. doi: 10.1016/0303-7207(78)90086-2.

Abstract

Populations of normal anterior pituitary cells enriched in thyrotrophs or mammotrophs prepared by velocity sedimentation were used to investigate the effect of modulators of TSH and prolactin secretion on cyclic AMP accumulation. In both thyrotroph-enriched and mammotroph-enriched fractions, IBMX increased cyclic AMP accumulation. In the presence of IBMX, TRH invoked an increase in cyclic AMP suggesting that TRH modulates cyclic AMP accumulation in both of these cell types from normal pituitary glands. In the mammotroph-rich fraction, dopamine inhibited the increase in cyclic AMP induced by TRH. In contrast however, in the thyrotroph-enriched fraction dopamine lowered neither cyclic AMP concentration nor TSH secretion. Thus the inhibiting effect of dopamine on cyclic AMP appears to be specific for prolactin-secreting cells.

摘要

通过速度沉降制备的富含促甲状腺激素细胞或催乳激素细胞的正常垂体前叶细胞群体，被用于研究促甲状腺激素和催乳激素分泌调节剂对环磷酸腺苷积累的影响。在富含促甲状腺激素细胞和富含催乳激素细胞的组分中，异丁基甲基黄嘌呤（IBMX）均增加了环磷酸腺苷的积累。在存在IBMX的情况下，促甲状腺激素释放激素（TRH）引起环磷酸腺苷增加，这表明TRH调节来自正常垂体的这两种细胞类型中环磷酸腺苷的积累。在富含催乳激素细胞的组分中，多巴胺抑制TRH诱导的环磷酸腺苷增加。然而，相比之下，在富含促甲状腺激素细胞的组分中，多巴胺既不降低环磷酸腺苷浓度，也不降低促甲状腺激素分泌。因此，多巴胺对环磷酸腺苷的抑制作用似乎对催乳激素分泌细胞具有特异性。