由大肠杆菌质粒pIE636编码的乙酰转移酶使诺尔斯菌素（链丝菌素）失活：乙酰基的位置
Nourseothricin (streptothricin) inactivated by a plasmid pIE636 encoded acetyl transferase of Escherichia coli: location of the acetyl group.
作者信息
Zähringer U, Voigt W, Seltmann G
机构信息
Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, FRG.
出版信息
FEMS Microbiol Lett. 1993 Jul 1;110(3):331-4. doi: 10.1111/j.1574-6968.1993.tb06344.x.
Escherichia coli strains harbouring the plasmid pIE636 are able to synthesize acetylcoenzyme A: streptothricin acetyltransferase (ACSAT). The (enzymatic) N-acetylation of streptothricin F is known to contribute significantly towards the loss of antibacterial activity. 13C-NMR analysis of [14C]N-acetyl-labelled streptothricin F, produced by ACSAT-catalysed acetylation of streptothricin F and subsequent purification by various chromatographical steps, unequivocally revealed streptothricin F to be acetylated at the beta-amino group (C16) (and not at the epsilon-amino group (C19)).
携带质粒pIE636的大肠杆菌菌株能够合成乙酰辅酶A：链丝菌素乙酰转移酶（ACSAT）。已知链丝菌素F的（酶促）N-乙酰化显著导致抗菌活性丧失。通过ACSAT催化链丝菌素F乙酰化并随后通过各种色谱步骤纯化产生的[14C]N-乙酰基标记的链丝菌素F的13C-NMR分析明确显示链丝菌素F在β-氨基（C16）处被乙酰化（而不是在ε-氨基（C19）处）。
Zotero 插件