Segondy M, Barat L, Mourad G, Huguet M F, Serre A, Vendrell J P
Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 249, University Hospital, Montpellier, France.
J Med Virol. 1993 Jul;40(3):200-3. doi: 10.1002/jmv.1890400306.
Cytomegalovirus-specific in vitro antibody production (CMV-IVAP) by peripheral blood lymphocytes (PBL) was investigated in 12 renal transplant recipients. CMV-IVAP, CMV-serology, and viral cultures were carried out weekly during at least 8 weeks after transplantation. Nine episodes of CMV infection occurred in eight patients. CMV-IVAP was positive in eight episodes; IgG and/or IgA and/or IgM antibodies reacting with several CMV polypeptides were secreted by PBL. In two patients with primary CMV infection, only IgA antibodies were detected. Cytomegalovirus cultures were positive in eight episodes and serological evidence of CMV infection was obtained in five episodes. In one case, CMV-IVAP was observed before CMV isolation and in another case, before serological evidence of CMV infection. Our study indicates that CMV-IVAP could represent an additional tool for the diagnosis of CMV infection. Our study indicates that CMV-IVAP could represent an additional tool for the diagnosis of CMV infection in renal transplant recipients.
对12例肾移植受者外周血淋巴细胞（PBL）产生巨细胞病毒特异性体外抗体（CMV-IVAP）的情况进行了研究。在移植后的至少8周内，每周进行CMV-IVAP、CMV血清学检测和病毒培养。8例患者发生了9次巨细胞病毒感染。8次感染中CMV-IVAP呈阳性；PBL分泌了与几种巨细胞病毒多肽发生反应的IgG和/或IgA和/或IgM抗体。在2例原发性巨细胞病毒感染患者中，仅检测到IgA抗体。8次感染中巨细胞病毒培养呈阳性，5次感染获得了巨细胞病毒感染的血清学证据。1例中，在巨细胞病毒分离前观察到CMV-IVAP阳性，另1例中，在获得巨细胞病毒感染的血清学证据前观察到CMV-IVAP阳性。我们的研究表明，CMV-IVAP可能是诊断肾移植受者巨细胞病毒感染的一种辅助手段。我们的研究表明，CMV-IVAP可能是诊断肾移植受者巨细胞病毒感染的一种辅助手段。
