Sarov I, Levy E, Aymard M, Chardonnet Y, Bosshard S, Revillard J P, Friedman M, Nord E, Greiff M, Haikin H
Clin Exp Immunol. 1982 May;48(2):321-8.
The feasibility of using human cytomegalovirus (CMV)-specific IgA antibody determinations as a signal for early detection of recurrent CMV infections in eight renal transplant recipients was analyzed. Solid phase radioimmunoassay (RIA), enzyme-liked immunosorbent assay (ELISA) and immunoperoxidase assay (IPA) techniques were used for IgA antibody determinations. In parallel, IgG antibodies to CMV were studied by immunoperoxidase assay. A significant rise of CMV-specific IgG antibody titre was observed in all of these patients between 5 and 53 weeks post-transplantation. CMV-specific IgA antibody production was detected close to the time a rise in CMV IgG antibody was observed in seven out of eight patients studied by RIA and ELISA, and in six out of eight patients studied by IPA. In two patients specific CMV IgA antibodies were detected by all three methods before a significant rise of CMV IgG antibody titre was demonstrated. In these patients CMV IgA was detected by RIA earlier than by ELISA and IPA. The potential application of CMV-specific IgA antibody determination for early detection of recurrent CMV infection in renal transplant patients is discussed.
分析了采用人巨细胞病毒（CMV）特异性IgA抗体检测作为8例肾移植受者复发性CMV感染早期检测信号的可行性。采用固相放射免疫测定法（RIA）、酶联免疫吸附测定法（ELISA）和免疫过氧化物酶测定法（IPA）检测IgA抗体。同时，采用免疫过氧化物酶测定法研究CMV的IgG抗体。在所有这些患者移植后5至53周期间，观察到CMV特异性IgG抗体滴度显著升高。通过RIA和ELISA研究的8例患者中有7例，通过IPA研究的8例患者中有6例，在观察到CMV IgG抗体升高的时间附近检测到CMV特异性IgA抗体产生。在2例患者中，在CMV IgG抗体滴度显著升高之前，所有三种方法均检测到特异性CMV IgA抗体。在这些患者中，RIA检测到CMV IgA比ELISA和IPA更早。讨论了CMV特异性IgA抗体检测在肾移植患者复发性CMV感染早期检测中的潜在应用。
