Mazzolli A B, Bustuoabad O D, Barrera C, Mancini R E
Int J Fertil. 1976;21(1):49-54.
Adult outbread male guinea pigs were autoimmunized without adjuvant. Homogenates were prepared with one of their own testes previously submitted "in vivo" and "in vitro" to thermal injury. Animals received a single or daily repeated intradermal injection without added adjuvant, in one or different skin sites. Guinea pigs daily sensitized in the same site during 30 days showed the presence of: a) dermal granuloma at the site of injection; b) several foci of typical allergic orchitis; c) delayed hypersensitivity detected by inhibition of macrophage migration; d) moderate titres of spermagglutinins and negligible levels of hemagglutinating antibodies. Guinea pigs receiving a single dose in one site only developed delayed hypersensitivity. Animals daily sensitized with the same dose of altered antigen in different sites, or with normal testis antigen either in one or different sites, showed negative results. The correlation among testicular lesion, dermal granuloma and cellular immunity is discussed. It is concluded that testis autosensitization is obtained in the absence of added adjuvant provided that a thermally injured gonad used as antigen is repeatedly injected in the same site.
成年远交系雄性豚鼠在无佐剂的情况下进行自身免疫。用其自身先前在“体内”和“体外”遭受热损伤的一个睾丸制备匀浆。动物在一个或不同皮肤部位接受单次或每日重复的无添加佐剂的皮内注射。在30天内每日在同一部位致敏的豚鼠表现出：a）注射部位的皮肤肉芽肿；b）几个典型过敏性睾丸炎病灶；c）通过巨噬细胞迁移抑制检测到的迟发型超敏反应；d）中等滴度的精子凝集素和可忽略不计的血凝抗体水平。仅在一个部位接受单剂量注射的豚鼠仅产生迟发型超敏反应。在不同部位每日用相同剂量的改变抗原致敏的动物，或在一个或不同部位用正常睾丸抗原致敏的动物，结果均为阴性。讨论了睾丸病变、皮肤肉芽肿和细胞免疫之间的相关性。得出的结论是，在无添加佐剂的情况下可实现睾丸自身致敏，前提是将用作抗原的热损伤性腺反复注射到同一部位。
