Adachi K, Watanabe T, Yamane S, Makimura S
Department of Veterinary Internal Medicine, Faculty of Agriculture, Miyazaki University, Japan.
J Vet Med Sci. 1993 Jun;55(3):487-90. doi: 10.1292/jvms.55.487.
To eliminate the influence of anti-dog erythrocyte membrane antibodies in Babesia gibsoni-infected dogs on the immunological investigations of parasites, an attempt was made to purify B. gibsoni piroplasms from infected erythrocytes by N2 cavitation and centrifugation in a Percoll discontinuous density gradient. Although electron microscopic examination revealed that the concentrated piroplasms visible as a band at the interface between 30% and 40% Percoll solutions were contaminated with a small amount of erythrocyte membrane debris, little contamination was detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western immunoblots. Hence, the isolated parasites may be usable for immunological examinations of parasites.
为消除吉氏巴贝斯虫感染犬体内抗犬红细胞膜抗体对寄生虫免疫学研究的影响，尝试通过N2空化作用以及在Percoll不连续密度梯度中离心，从感染的红细胞中纯化吉氏巴贝斯虫梨形虫。尽管电子显微镜检查显示，在30%和40%Percoll溶液界面处可见的浓缩梨形虫带有少量红细胞膜碎片污染，但十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和Western免疫印迹检测到的污染很少。因此，分离出的寄生虫可用于寄生虫的免疫学检查。
