灵长类动物肾脏中内源性蛋白质的磷酸化：钙/钙调蛋白依赖性激酶调节剂的作用

Phosphorylation of endogenous protein in primate kidney: effects of modulators of calcium/calmodulin-dependent kinases.

Laminski N A, Hammond K D, Meyers A M

Department of Medical Biochemistry, University of the Witwatersrand, Johannesburg, South Africa.

Comp Biochem Physiol B. 1993 Jun;105(2):415-20. doi: 10.1016/0305-0491(93)90250-9.

Abstract

Phosphorylation of endogenous proteins in response to calcium and calmodulin was assessed in membrane and cytosol from primate kidney. 2. Quantitative studies showed that calcium and calmodulin had little effect on phosphorylation in baboon kidney cytosol; in membranes, phosphorylation was significantly decreased by calcium and calmodulin. 3. Phosphorylation of specific proteins which had been electrophoretically separated indicated that calmodulin, in the absence or presence of calcium, enhanced bands of masses 37.1 +/- 1.1 kDa and 103.3 +/- 6.2 kDa (N = 6) in cytosol fractions of baboon kidney; in membrane fractions no effect was noted. 4. Similar results were found in normal human kidney.

摘要

在灵长类动物肾脏的细胞膜和细胞溶质中，评估了内源性蛋白质对钙和钙调蛋白的磷酸化反应。2. 定量研究表明，钙和钙调蛋白对狒狒肾脏细胞溶质中的磷酸化作用很小；在细胞膜中，钙和钙调蛋白可使磷酸化显著减少。3. 对经电泳分离的特定蛋白质的磷酸化研究表明，无论有无钙存在，钙调蛋白均可增强狒狒肾脏细胞溶质组分中质量为37.1±1.1 kDa和103.3±6.2 kDa的条带（N = 6）；在细胞膜组分中未观察到影响。4. 在正常人类肾脏中也发现了类似结果。