Victor K D, Pascual V, Stitzel A E, Tsokos G C, Capra J D, Spitzer R E
University of Texas Southwestern Medical Center, Deparmtent of Microbiology, Dallas 75235-9048.
Hybridoma. 1993 Jun;12(3):231-7. doi: 10.1089/hyb.1993.12.231.
The production of autoantibodies to the alternative pathway C3/C5 convertase or C3 Nephritic Factor (C3NeF) is one characteristic of membranoproliferative glomerulonephritis. The complete nucleotide sequences of the heavy and light chain variable regions of an IgG C3NeF produced by an EBV transformed B cell line derived from a patient with membranoproliferative glomerulonephritis were determined. The VH and VL gene segments used by this C3NeF are extensively mutated suggesting that antigenic selection and affinity maturation may occur during the generation of these autoantibodies.
产生针对替代途径C3/C5转化酶的自身抗体或C3肾炎因子（C3NeF）是膜增生性肾小球肾炎的一个特征。已确定了一名膜增生性肾小球肾炎患者来源的EBV转化B细胞系产生的IgG C3NeF重链和轻链可变区的完整核苷酸序列。该C3NeF所使用的VH和VL基因片段发生了广泛突变，这表明在这些自身抗体的产生过程中可能发生了抗原选择和亲和力成熟。
