Oultram J D, Burr I D, Elmore M J, Minton N P
Division of Biotechnology, PHLS Centre for Applied Microbiology and Research, Salisbury, Wilts, UK.
Gene. 1993 Sep 6;131(1):107-12. doi: 10.1016/0378-1119(93)90677-u.
An 8.1-kb fragment of chromosomal DNA from Clostridium acetobutylicum NCIMB 8052 (formerly NCIB 8052) has been cloned into plasmid pAT153 and shown to allow the growth of Escherichia coli LJ32 (F+ atoC2c atoD32 fadR) on butyrate as the sole source of carbon and energy. Deletion analysis delineated a 3.9-kb subfragment capable of complementation. The nucleotide sequence of this fragment was determined and it was shown to encode three complete, and two incomplete open reading frames (ORFs). Based on enzymic studies of recombinant clones, two of these ORFs were shown to encode phosphotransbutyrylase and butyrate kinase. The above enzymes are involved in the acidogenic phase of fermentation in C. acetobutylicum. The fragment also carries an incomplete ORF encoding a polypeptide exhibiting substantial similarity to dihydropteroate synthase.
来自丙酮丁醇梭菌NCIMB 8052（原NCIB 8052）的一段8.1 kb染色体DNA片段已被克隆到质粒pAT153中，并显示其能使大肠杆菌LJ32（F+ atoC2c atoD32 fadR）以丁酸盐作为唯一碳源和能源生长。缺失分析确定了一个能够互补的3.9 kb亚片段。测定了该片段的核苷酸序列，结果表明它编码三个完整的和两个不完整的开放阅读框（ORF）。基于对重组克隆的酶学研究，其中两个ORF被证明编码磷酸转丁酰酶和丁酸激酶。上述酶参与丙酮丁醇梭菌发酵的产酸阶段。该片段还带有一个不完整的ORF，其编码的多肽与二氢蝶酸合酶具有显著相似性。
