Bartlett D H, Chi E, Wright M E
Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla 92093-0202.
Gene. 1993 Sep 6;131(1):125-8. doi: 10.1016/0378-1119(93)90680-2.
In contrast to studies of many other extremophiles, the molecular characterization of the barophilic or high-pressure-adapted bacteria of the deep ocean is virtually nonexistent. One exception is the discovery that the moderate barophile Photobacterium SS9 preferentially synthesizes a 37-kDa outer membrane protein, designated OmpH, in response to elevated hydrostatic pressure. We report here on the molecular characterization of the ompH gene. The deduced amino acid sequence of mature OmpH is similar to a number of porin proteins, including significant similarity to porin protein P2 from Haemophilus influenzae. It appears likely that OmpH is a unique porin whose synthesis is responsive to changes in the pressure regime of the deep-sea bacterium.
与许多其他嗜极生物的研究不同，对深海嗜压或高压适应细菌的分子特征研究几乎不存在。一个例外是发现中度嗜压菌费氏弧菌SS9在静水压力升高时会优先合成一种37 kDa的外膜蛋白，命名为OmpH。我们在此报告ompH基因的分子特征。成熟OmpH推导的氨基酸序列与许多孔蛋白相似，包括与流感嗜血杆菌的孔蛋白P2有显著相似性。OmpH似乎是一种独特的孔蛋白，其合成对深海细菌压力环境的变化有反应。
