Wu Y L, Lee L H, Rollins D M, Ching W M
Naval Medical Research Institute, Bethesda, Maryland 20889-5607.
Infect Immun. 1994 Oct;62(10):4256-60. doi: 10.1128/iai.62.10.4256-4260.1994.
The protein response to physiological stress was characterized in Campylobacter jejuni 81176 after exposure to heat and pH shock and following periods of recovery. Immunoreactivities of major stress-related proteins were determined with anti-Campylobacter immune rabbit serum and intestinal lavage fluid. Distinct proteins with molecular masses ranging from 10 to 120 kDa were induced and/or released by selective heat or pH treatments. The most notable responses were those of two proteins with apparent molecular masses of 45 and 64 kDa that were induced and two other proteins of 10 and 12 kDa that were released by selective heat shock, alkaline pH treatment, or both. On the basis of N-terminal sequence analysis and immunological cross-reactivity data, the 64- and 10-kDa proteins were the C. jejuni homologs of Escherichia coli GroEL and GroES proteins, respectively. Enhanced chemiluminescence Western blotting (immunoblotting) revealed that all four proteins were among the major protein antigens recognized by anti-Campylobacter rabbit serum immunoglobulin G (IgG) and immune rabbit intestinal lavage IgA (secretory IgA). The results of this investigation suggest that the C. jejuni 10-, 12-, 45-, and 64-kDa proteins and a number of minor stress-related proteins deserve further evaluation of their respective roles in Campylobacter pathogenesis and immunity.
在空肠弯曲菌81176暴露于热应激和pH应激以及恢复一段时间后，对其蛋白质对生理应激的反应进行了表征。使用抗弯曲菌免疫兔血清和肠灌洗液测定主要应激相关蛋白的免疫反应性。通过选择性热或pH处理诱导和/或释放了分子量范围为10至120 kDa的不同蛋白质。最显著的反应是两种表观分子量为45和64 kDa的蛋白质被诱导，以及另外两种分子量为10和12 kDa的蛋白质通过选择性热休克、碱性pH处理或两者同时处理而被释放。基于N端序列分析和免疫交叉反应数据，64 kDa和10 kDa的蛋白质分别是空肠弯曲菌中大肠杆菌GroEL和GroES蛋白的同源物。增强化学发光免疫印迹法（免疫印迹）显示，这四种蛋白质都是抗弯曲菌兔血清免疫球蛋白G（IgG）和免疫兔肠灌洗液IgA（分泌型IgA）识别的主要蛋白质抗原。本研究结果表明，空肠弯曲菌的10 kDa、12 kDa、45 kDa和64 kDa蛋白质以及一些次要的应激相关蛋白质在弯曲菌发病机制和免疫中的各自作用值得进一步评估。
