编码泛素融合蛋白的硕大利什曼原虫基因序列。

Sequence of a Leishmania major gene encoding an ubiquitin fusion protein.

作者信息

Graeff G R, Steele P M, Peterson C L, Bennett M L, Langer P J

机构信息

Department of Molecular Biology, University of Wyoming, Laramie 82071-3944.

出版信息

Gene. 1993 Sep 6;131(1):155-6. doi: 10.1016/0378-1119(93)90687-x.

DOI:10.1016/0378-1119(93)90687-x

PMID:8396548

Abstract

A gene encoding an ubiquitin-tail protein fusion was isolated from the parasitic protozoan, Leishmania major, and sequenced. The L. major tail protein shares 97, 96, 67, 62, 62 and 61% sequence identity with the tail proteins of Trypanosoma brucei, Trypanosoma cruzi, yeast, Dictyostelium discoideum, human, and Arabidopsis thaliana, respectively. The putative 'zinc finger' nucleic acid-binding domain found in all ubiquitin 'tail' or 'extension' proteins described is also conserved in the L. major sequence. The upstream sequence indicated that this gene is not located at the end of a polyubiquitin sequence.

摘要

从寄生原生动物硕大利什曼原虫中分离出一个编码泛素尾蛋白融合体的基因并进行了测序。硕大利什曼原虫的尾蛋白与布氏锥虫、克氏锥虫、酵母、盘基网柄菌、人类和拟南芥的尾蛋白序列同一性分别为97%、96%、67%、62%、62%和61%。在所有已描述的泛素“尾”或“延伸”蛋白中发现的推定“锌指”核酸结合结构域在硕大利什曼原虫序列中也保守。上游序列表明该基因并不位于多聚泛素序列的末端。