Searle S, Smith D F
Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, U.K.
Exp Parasitol. 1993 Aug;77(1):43-52. doi: 10.1006/expr.1993.1059.
The DNA sequence of a single-copy gene from Leishmania major has been determined and shown to share sequence identity with eukaryotic heat shock protein-70-related genes. Conserved features of the deduced open reading frame include amino acids implicated in ATP binding and a putative calmodulin-binding domain. Antibodies generated to the recombinant fusion protein recognise a 70-kDa molecule of pI 6.0. This molecule is constitutively expressed and localises to the cytoplasm in all stages of the parasite life cycle.
已经确定了来自硕大利什曼原虫的一个单拷贝基因的DNA序列，并显示其与真核热休克蛋白-70相关基因具有序列同一性。推导的开放阅读框的保守特征包括与ATP结合有关的氨基酸和一个假定的钙调蛋白结合结构域。针对重组融合蛋白产生的抗体识别出一个分子量为70 kDa、等电点为6.0的分子。该分子组成性表达，并定位于寄生虫生命周期所有阶段的细胞质中。
