Glasner H
Klin Wochenschr. 1977 Feb 15;55(4):181-7. doi: 10.1007/BF01469139.
This paper reports on a method which allows the separation of proteins of non concentrated CSF by means of the microzone technic. Pherograms of high quality were possible when the CSF contained more than 10 mg/dl total protein. Protein separation is completed in 16 min when one cellulose acetate strip with 150 V and 2 mA or when 150 V and 4 mA with two strips are used. Proteins were stained with nigrosine. The theoretical basis, technical details and error range of this method of protein separation are discussed.
本文报道了一种通过微区技术分离未浓缩脑脊液中蛋白质的方法。当脑脊液中总蛋白含量超过10mg/dl时，可获得高质量的层析图谱。使用一条醋酸纤维素条带，电压150V、电流2mA，或使用两条条带，电压150V、电流4mA时，16分钟即可完成蛋白质分离。蛋白质用苯胺黑染色。讨论了这种蛋白质分离方法的理论基础、技术细节和误差范围。
