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未浓缩脑脊液的简易微区电泳及浓缩与未浓缩脑脊液的琼脂凝胶电泳的诊断价值

Diagnostic value of brief microzone electrophoresis of unconcentrated CSF and agar gel electrophoresis of concentrated and unconcentrated CSF.

作者信息

Glasner H, Lowenthal A, Karcher D

出版信息

J Neurol. 1979;222(1):53-8. doi: 10.1007/BF00313267.

DOI:10.1007/BF00313267
PMID:93627
Abstract

This paper reports a comparison between brief microzone electrophoresis and agar gel electrophoresis of CSF proteins; 41 different CSFs were examined simultaneously by all three methods. Fractionation of gamma-globulins was found by brief microzone electrophoresis in 53.7% of unconcentrated CSF. This fractionation was found in 63.4% of unconcentrated CSF after agar gel electrophoresis, in 48.8% of concentrated CSF. Serum-like pherogram and the rapid alpha-globulin type were found equally often.

摘要

本文报告了脑脊液蛋白的简易微区电泳与琼脂凝胶电泳之间的比较;采用这三种方法同时检测了41份不同的脑脊液样本。在53.7%的未浓缩脑脊液中,通过简易微区电泳发现了γ-球蛋白的分级分离现象。琼脂凝胶电泳后,在63.4%的未浓缩脑脊液中发现了这种分级分离现象,在48.8%的浓缩脑脊液中也有发现。血清样图谱和快速α-球蛋白类型出现的频率相同。

相似文献

1
Diagnostic value of brief microzone electrophoresis of unconcentrated CSF and agar gel electrophoresis of concentrated and unconcentrated CSF.未浓缩脑脊液的简易微区电泳及浓缩与未浓缩脑脊液的琼脂凝胶电泳的诊断价值
J Neurol. 1979;222(1):53-8. doi: 10.1007/BF00313267.
2
Microzone electrophoresis of the unconcentrated and concentrated CSF.未浓缩和浓缩脑脊液的微量区带电泳。
J Neurol. 1978 Apr 14;218(1):73-6. doi: 10.1007/BF00314721.
3
Agar gel electrophoresis of unconcentrated cerebrospinal fluid: the degenerative type.未浓缩脑脊液的琼脂凝胶电泳:退化型。
Acta Neurol Belg. 1974 Nov-Dec;74(6):376-82.
4
[Micro-electrophoresis of unconcentrated cerebrospinal fluid (author's transl)].[未浓缩脑脊液的微电泳法(作者译)]
Dtsch Med Wochenschr. 1978 Aug 11;103(32):1274-6. doi: 10.1055/s-0028-1129246.
5
Oligoclonal bands in cerebrospinal fluid detected by PhastSystem isoelectric focusing.通过PhastSystem等电聚焦法检测脑脊液中的寡克隆带。
Clin Chem. 1990 Jan;36(1):123-5.
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[Diagnostic value of microzone electrophoresis of non-concentrated cerebrospinal fluid (author's transl)].非浓缩脑脊液微区电泳的诊断价值(作者译)
Dtsch Med Wochenschr. 1978 Jul 21;103(29):1173-6. doi: 10.1055/s-0028-1129224.
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Agarose gel electrophoresis of cerebrospinal fluid proteins and analysis of the pherogram profiles by analog computer.脑脊液蛋白质的琼脂糖凝胶电泳及通过模拟计算机分析电泳图谱。
J Clin Chem Clin Biochem. 1977 Nov;15(11):635-44. doi: 10.1515/cclm.1977.15.1-12.635.
8
[Microzone electrophoresis of non concentrated CSF (author's transl)].非浓缩脑脊液的微区电泳(作者译)
Klin Wochenschr. 1977 Feb 15;55(4):181-7. doi: 10.1007/BF01469139.
9
Effects of two radiocontrast dyes on the detection of oligoclonal gamma globulins by high resolution agarose gel electrophoresis.两种放射性造影剂对高分辨率琼脂糖凝胶电泳检测寡克隆γ球蛋白的影响。
Am J Clin Pathol. 1983 May;79(5):607-8. doi: 10.1093/ajcp/79.5.607.
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Analytical isotachophoresis: a new method for analysis of cerebrospinal fluid proteins. A preliminary report.分析等速电泳法:一种分析脑脊液蛋白质的新方法。初步报告。
J Neurol Sci. 1975 Dec;26(4):617-22. doi: 10.1016/0022-510x(75)90064-7.

本文引用的文献

1
Micro-electrophoresis in agar gel of proteins of the cerebrospinal fluid and central nervous system.脑脊液和中枢神经系统蛋白质的琼脂凝胶微电泳
J Neurochem. 1959 Jun;4(2):135-40. doi: 10.1111/j.1471-4159.1959.tb13182.x.
2
[A new micro-electrophoretic proceedure for untreated liquor cerebrospinalis. Differences in the pherogram of native and concentrated proteins. Studies on the protein electrophoresis of liquor cerebrospinalis with cellulose acetate membranes, II (author's transl)].[一种用于未经处理的脑脊液的新型微电泳方法。天然蛋白和浓缩蛋白电泳图谱的差异。用醋酸纤维素膜对脑脊液进行蛋白质电泳的研究,II(作者译)]
Z Klin Chem Klin Biochem. 1974 Feb;12(2):73-80.
3
[The significance of discontinuous zonation of electrophoretically separated gamma globulins for the diagnosis of neurological diseases (author's transl)].
[电泳分离的γ球蛋白的不连续区带对神经系统疾病诊断的意义(作者译)]
Z Neurol. 1973 Dec 31;206(1):25-38.
4
A highly sensitive method for demonstrating proteins in electrophoretic, immunoelectrophoretic and immunodiffusion preparations.一种用于在电泳、免疫电泳和免疫扩散制剂中检测蛋白质的高灵敏度方法。
Clin Chim Acta. 1972 May;38(2):465-7. doi: 10.1016/0009-8981(72)90142-8.
5
Agargel electrophoresis of unconcentrated cerebrospinal fluid.未浓缩脑脊液的琼脂糖凝胶电泳。
J Neurol. 1975;209(1):59-63. doi: 10.1007/BF00312526.