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Inhibition of human cytomegalovirus major immediate early gene expression by antisense RNA expression vectors.

作者信息

Bryant L A, Sinclair J H

机构信息

Department of Medicine, University of Cambridge, Addenbrooke's Hospital, U.K.

出版信息

J Gen Virol. 1993 Sep;74 ( Pt 9):1965-7. doi: 10.1099/0022-1317-74-9-1965.

DOI:10.1099/0022-1317-74-9-1965
PMID:8397287
Abstract

We have used antisense oligonucleotides and expression vectors to inhibit human cytomegalovirus (HCMV) major immediate early (IE) gene expression. We find that oligonucleotides complementary to the HCMV 72K IE protein (IE1) coding region do inhibit HCMV infection, but this is non-specific. However, the use of certain antisense expression vectors, which express short oligonucleotides complementary to IE1, specifically inhibits IE1 expression at the protein level after introduction of IE expression vectors into cells or after HCMV infection.

摘要

相似文献

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Inhibition of human cytomegalovirus major immediate early gene expression by antisense RNA expression vectors.
J Gen Virol. 1993 Sep;74 ( Pt 9):1965-7. doi: 10.1099/0022-1317-74-9-1965.
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