Zerlin M, Julius M A, Goldfarb M
Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Oncogene. 1993 Oct;8(10):2731-9.
We have isolated a murine cDNA, nep, which encodes a novel receptor-like protein tyrosine kinase. The kinase region of NEP protein bears 50% amino acid sequence identity to the neurotrophin receptors (TRKs). While the intracytoplasmic portion of NEP also contains a short kinase insert region and C-terminal tail reminiscent of the TRK proteins, the putative extracellular domain of NEP is unrelated to any known proteins. The nep gene is strongly expressed within proliferating neuroepithelia of mouse embryos, commencing at the early somite stage (embryonic day 8.0) and persisting in the proliferative ventricular zones of the brain and spinal cord, suggesting that one function of NEP kinase is to signal proliferation of neuroepithelial cells in response to an as yet unknown ligand. The nep gene is also expressed in embryonic sensory ganglia, striated muscle and epidermis, as well as in several adult tissues, including the ventricle linings and glia subpopulations in the brain.
我们分离出了一个小鼠cDNA，即nep，它编码一种新型的受体样蛋白酪氨酸激酶。NEP蛋白的激酶区域与神经营养因子受体（TRKs）的氨基酸序列有50%的同一性。虽然NEP的胞质部分也包含一个短的激酶插入区域和类似于TRK蛋白的C末端尾巴，但NEP的假定细胞外结构域与任何已知蛋白质都不相关。nep基因在小鼠胚胎增殖的神经上皮中强烈表达，从早期体节阶段（胚胎第8.0天）开始，并持续存在于脑和脊髓的增殖脑室区，这表明NEP激酶的一个功能是响应一种未知配体来信号传导神经上皮细胞的增殖。nep基因也在胚胎感觉神经节、横纹肌和表皮中表达，以及在几种成年组织中表达，包括脑室衬里和脑中的胶质细胞亚群。
