Auger M, Katz R L, Johnston D A, Sneige N, Ordonez N G, Fritsche H
Department of Anatomic Pathology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Anal Quant Cytol Histol. 1993 Aug;15(4):274-80.
Evaluation of estrogen receptors (ER) and progesterone receptors (PR) in breast carcinoma using ER and PR immunocytochemical assays (ER-ICA and PR-ICA) has traditionally been semiquantitative at best. We investigated the use of the SAMBA 4000 image analysis system for quantitation of immunocytochemical ER content in 23 cases and PR content in 20 cases of breast carcinoma after obtaining cytology specimens by fine needle aspiration (FNA). We compared the quantitative assessment obtained by image analysis, the quick score (QS), to the biochemical assay as well as to the ER-ICA and PR-ICA semiquantitative visual histochemical score (H-score). Using Pearson's linear correlation coefficient, we demonstrated that for both ER and PR, the QS obtained by the "area" or "nuclear" programs correlated significantly with the H-scores. However, while the QS obtained on the area program for both ER and PR correlated significantly with the biochemical assay, the QS obtained on the nuclear program did not. The discrepancy might reflect the smaller number of cells evaluated with the nuclear program as compared to the area one. These results show that the SAMBA 4000 system provides an objective means of quantitating ER and PR content by immunocytochemical methods.
传统上，使用雌激素受体（ER）和孕激素受体（PR）免疫细胞化学检测法（ER-ICA和PR-ICA）对乳腺癌中的ER和PR进行评估充其量只是半定量的。在通过细针穿刺（FNA）获取细胞学标本后，我们研究了使用SAMBA 4000图像分析系统对23例乳腺癌中的免疫细胞化学ER含量和20例乳腺癌中的PR含量进行定量分析。我们将通过图像分析获得的定量评估结果——快速评分（QS），与生化检测结果以及ER-ICA和PR-ICA半定量视觉组织化学评分（H评分）进行了比较。通过Pearson线性相关系数，我们证明，对于ER和PR而言，通过“面积”或“细胞核”程序获得的QS与H评分显著相关。然而，虽然通过面积程序获得的ER和PR的QS与生化检测结果显著相关，但通过细胞核程序获得的QS却并非如此。这种差异可能反映出与面积程序相比，细胞核程序所评估的细胞数量较少。这些结果表明，SAMBA 4000系统提供了一种通过免疫细胞化学方法对ER和PR含量进行定量分析的客观手段。
