Juarros E, Tortajada C, García M D, Uruburu F
Departamento de Microbiología, Facultad de Biológicas, Universitat de Valencia, Spain.
Microbiologia. 1993 Apr;9(1):28-33.
Freezing and storage at -80 degrees C has been applied to the preservation of nonsporulated filamentous fungi Phytophthora, Pythium, Sclerotinia and Rhizoctonia, and the results are presented. We had tested different methods of freezing and thawing, finding that the best results were obtained pre-cooling at 4 degrees C during 1 hour followed by freezing at -80 degrees C. The best thawing method was achieved at 37 degrees C. The technique was found to be simple and reliable for the culture collections labours of fungi maintenance.
-80℃冷冻保存已应用于非孢子化丝状真菌疫霉属、腐霉属、核盘菌属和丝核菌属的保存，并展示了相关结果。我们测试了不同的冻融方法，发现先在4℃预冷1小时，然后在-80℃冷冻可获得最佳效果。最佳解冻方法是在37℃解冻。该技术对于真菌保藏实验室的真菌保存工作而言简单可靠。
