头颈部癌患者肿瘤相关淋巴细胞的自体肿瘤细胞杀伤活性

Autologous tumor cell killing activity of tumor-associated lymphocytes in patients with head and neck carcinomas.

作者信息

Tsukuda M, Mochimatsu I, Sakumoto M, Furukawa S, Yuyama S, Yanoma S, Kubota A

机构信息

Department of Otorhinolaryngology. School of Medicine, Yokohama City University, Kanagawa, Japan.

出版信息

Biotherapy. 1993;6(2):155-61. doi: 10.1007/BF01877429.

DOI:10.1007/BF01877429

PMID:8398575

Abstract

In order to select the most cytotoxic effector cells for adoptive immunotherapy, lymphokine activated killer (LAK) cells, tumor infiltrating lymphocytes (TILs) and autologous mixed lymphocyte tumor cell culture (MLTC) cells derived from peripheral blood mononuclear cells (PBMC) in the same subject with head and neck carcinomas were prepared. The autologous tumor cell killing activity and cell surface phenotypes of each of the three effector cells were studied. MLTC cells cultured with interleukin-2 (IL-2) showed the strongest cytotoxic activity among these three different effector cells. Although TILs had suppressed killing activity immediately after isolation, after successive cultivations with IL-2, a cytotoxic activity against autologous tumor cells stronger than that of LAK cells appeared. Both IL-2 stimulated MLTC cells and TILs showed an enrichment of CD8 positive and CD11 negative cells in a CD3 positive subpopulation.

摘要

为了选择用于过继性免疫治疗的最具细胞毒性的效应细胞，制备了来自同一头颈癌患者外周血单个核细胞（PBMC）的淋巴因子激活的杀伤（LAK）细胞、肿瘤浸润淋巴细胞（TIL）和自体混合淋巴细胞肿瘤细胞培养（MLTC）细胞。研究了这三种效应细胞各自的自体肿瘤细胞杀伤活性和细胞表面表型。用白细胞介素-2（IL-2）培养的MLTC细胞在这三种不同的效应细胞中表现出最强的细胞毒性活性。尽管TIL在分离后立即具有抑制的杀伤活性，但在用IL-2连续培养后，出现了比LAK细胞更强的针对自体肿瘤细胞的细胞毒性活性。IL-2刺激的MLTC细胞和TIL在CD3阳性亚群中均显示CD8阳性和CD11阴性细胞富集。