白细胞介素2激活的肿瘤浸润淋巴细胞的功能和表型分析

Functional and phenotypic analyses of interleukin 2-activated tumor-infiltrating lymphocytes.

作者信息

Yamaue H, Tanimura H, Tsunoda T, Iwahashi M, Tani M, Tamai M, Inoue M

机构信息

Department of Gastroenterological Surgery, Wakayama Medical College, Japan.

出版信息

Biotherapy. 1990;2(3):247-59. doi: 10.1007/BF02173526.

DOI:10.1007/BF02173526

PMID:1698423

Abstract

The tumor-infiltrating lymphocytes (TILs) were cultured with interleukin 2 (IL-2) to induce the activated killer cells possessing autologous tumor-killing activity, and analysed their cell surface phenotypes and assessed anti-tumor killing activity. Furthermore, the activated TILs were transferred into 7 patients adoptively resulting in complete remission in a patient with pancreatic cancer and partial remission in another patient with gastric cancer. The cytotoxic activities of activated TILs at 3 weeks-incubation was 72 +/- 15, 42 +/- 26, 27 +/- 21 and 25 +/- 15% against K562, Daudi, KATO-III and autologous tumor, respectively. The negative selection method, indicated that the killer cells recognizing autologous tumor cells consisted of CD4- or CD8-positive T lymphocytes and CD16- or CD56-positive natural killer cells. The activated TILs could not only lyse cultured tumor cell lines, but also autologous tumor cells.

摘要

将肿瘤浸润淋巴细胞（TILs）与白细胞介素2（IL-2）一起培养，以诱导具有自体肿瘤杀伤活性的活化杀伤细胞，并分析其细胞表面表型并评估抗肿瘤杀伤活性。此外，将活化的TILs过继转移至7例患者，其中1例胰腺癌患者完全缓解，另1例胃癌患者部分缓解。培养3周的活化TILs对K562、Daudi、KATO-III和自体肿瘤的细胞毒性活性分别为72±15%、42±26%、27±21%和25±15%。阴性选择法表明，识别自体肿瘤细胞的杀伤细胞由CD4或CD8阳性T淋巴细胞以及CD16或CD56阳性自然杀伤细胞组成。活化的TILs不仅可以裂解培养的肿瘤细胞系，还可以裂解自体肿瘤细胞。