Minami T, Tojo H, Shinomura Y, Matsuzawa Y, Okamoto M
Second Department of Internal Medicine, Osaka University Medical School, Suita, Japan.
Biochim Biophys Acta. 1993 Oct 13;1170(2):125-30.
We purified a phospholipase A2 (PLA2) from human ileal mucosa to homogeneity. Its NH2-terminal amino acid sequence, amino acid composition, molecular weight, and elution behavior on reverse phase high-performance liquid chromatography were identical to those of human group II PLA2 purified from synovial fluid or spleen. The ileal PLA2 preferred anionic phosphatidylglycerol as substrate. On immunoblot analysis, human ileal mucosa gave more intense immunoreactivity with anti-human synovial fluid PLA2 antibody, at the same position as the purified enzyme, than the cecal mucosa. Northern blot analysis also showed that the level of group II PLA2 mRNA in the ileal mucosa was greater than that in the cecal mucosa. The enzyme was rather uniformly distributed over the colonic mucosa, from cecum to sigmoid colon. These results indicate that the ileal mucosa contains group II PLA2, and that its expression in the ileal mucosa was higher than that in the colonic mucosa.
我们从人回肠黏膜中纯化出一种磷脂酶A2（PLA2），使其达到同质。其氨基末端氨基酸序列、氨基酸组成、分子量以及在反相高效液相色谱上的洗脱行为，均与从滑液或脾脏中纯化出的人II型PLA2相同。回肠PLA2优先选择阴离子磷脂酰甘油作为底物。在免疫印迹分析中，与人滑液PLA2抗体相比，人回肠黏膜在与纯化酶相同的位置呈现出更强的免疫反应性，而盲肠黏膜则不然。Northern印迹分析还表明，回肠黏膜中II型PLA2 mRNA的水平高于盲肠黏膜。该酶在结肠黏膜中分布较为均匀，从盲肠到乙状结肠均有分布。这些结果表明，回肠黏膜含有II型PLA2，且其在回肠黏膜中的表达高于结肠黏膜。
