克罗恩病和溃疡性结肠炎患者结肠黏膜中Ⅱ型磷脂酶A2增加。
Increased group II phospholipase A2 in colonic mucosa of patients with Crohn's disease and ulcerative colitis.
作者信息
Minami T, Tojo H, Shinomura Y, Matsuzawa Y, Okamoto M
机构信息
Second Department of Internal Medicine, University Medical School, Japan.
出版信息
Gut. 1994 Nov;35(11):1593-8. doi: 10.1136/gut.35.11.1593.
The immunochemical protein content of group II phospholipase A2 (PLA2) and PLA2 enzymatic activity were measured for colonic mucosal biopsy samples obtained from patients with either Crohn's disease of the colon or ulcerative colitis, and control patients without inflammatory bowel disease. Immunoreactive group II PLA2 (IR-PLA2 II) content and PLA2 activity in actively inflamed colonic mucosa of Crohn's disease patients were significantly higher than those in inactively inflamed mucosa of Crohn's disease patients and the colonic mucosa of controls. IR-PLA2 II content and PLA2 activity in severely inflamed mucosa of ulcerative colitis patients were significantly higher than those in the colonic mucosa of the controls. Mucosal PLA2 enzymatic activity was closely correlated with mucosal IR-PLA2 II content in patients with Crohn's disease and ulcerative colitis. These results suggest that an increase in PLA2 enzymatic activity in inflamed colonic mucosa of Crohn's disease and ulcerative colitis was mainly attributed to increased protein content of group II PLA2, and that an increase in mucosal group II PLA2 may be involved in the pathogenesis of intestinal inflammation of Crohn's disease and ulcerative colitis.
对取自患有结肠克罗恩病或溃疡性结肠炎的患者以及无炎症性肠病的对照患者的结肠黏膜活检样本，测量了II型磷脂酶A2（PLA2）的免疫化学蛋白质含量和PLA2酶活性。克罗恩病患者活动期炎症结肠黏膜中的免疫反应性II型PLA2（IR-PLA2 II）含量和PLA2活性显著高于克罗恩病患者非活动期炎症黏膜及对照患者的结肠黏膜。溃疡性结肠炎患者严重炎症黏膜中的IR-PLA2 II含量和PLA2活性显著高于对照患者的结肠黏膜。在克罗恩病和溃疡性结肠炎患者中，黏膜PLA2酶活性与黏膜IR-PLA2 II含量密切相关。这些结果表明，克罗恩病和溃疡性结肠炎炎症结肠黏膜中PLA2酶活性的增加主要归因于II型PLA2蛋白质含量的增加，并且黏膜II型PLA2的增加可能参与了克罗恩病和溃疡性结肠炎肠道炎症的发病机制。
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