Gagnon R F, Richards G K, Subang R
Montreal General Hospital Research Institute, Canada.
Perit Dial Int. 1993;13 Suppl 2:S310-2.
Catheter-related sepsis, principally with S. epidermidis, remains a main complication of continuous ambulatory peritoneal dialysis (CAPD). A possible reason for the antibiotic resistance often displayed by these infections is the presence of bacteria growing in a protective biofilm on the catheter surface. We developed a reproducible stable model of experimental peritoneal catheter-associated infection with S. epidermidis in the mouse and used this model to examine the therapeutic efficacy of vancomycin. The response to vancomycin treatment given daily (15 mg/kg body weight) for periods from 1-14 days, relating the proportion of successful outcome (sterilization of implant infection) over time, was typical of an S-shaped biological response curve. These results extend our previous observations in vitro of the activity of vancomycin against S. epidermidis biofilm preparations and serve as a rational basis for the experimental evaluation of synergy and antagonism in the treatment of implant-associated infection.
导管相关败血症，主要由表皮葡萄球菌引起，仍然是持续性非卧床腹膜透析（CAPD）的主要并发症。这些感染常常表现出抗生素耐药性，一个可能的原因是细菌在导管表面形成保护性生物膜生长。我们建立了一种可重复的、稳定的小鼠实验性腹膜导管相关表皮葡萄球菌感染模型，并使用该模型研究万古霉素的治疗效果。每天给予万古霉素治疗（15毫克/千克体重），持续1至14天，随着时间推移，成功治疗结果（植入物感染除菌）的比例呈现出典型的S形生物学反应曲线。这些结果扩展了我们之前在体外对万古霉素抗表皮葡萄球菌生物膜制剂活性的观察，并为植入物相关感染治疗中协同作用和拮抗作用的实验评估提供了合理依据。
