Schultes B C, Reinsberg J, Schlebusch H, Oehr P, Biersack H J, Krebs D, Wagner U
Zentrum für Geburtshilfe und Frauenheilkunde, Universität Bonn, Deutschland.
Eur J Clin Chem Clin Biochem. 1993 Jul;31(7):427-32. doi: 10.1515/cclm.1993.31.7.427.
By immunization of mice with the anti-CA 125 monoclonal antibody OC125, we tried to induce antibodies directed against the tumour-associated antigen CA125, via activation of the idiotypic network. Mice immunized by repeated administrations of F(ab')2-fragments of the OC125 antibody produced anti-idiotypic antibodies, imitating the original target antigen of the OC125. After induction of these anti-idiotypic antibodies (Ab2 beta) a murine IgG-anti-CA125 (Ab3) response was detected. The induction of idiotypic cascades offers the possibility of immunization against tumour-associated antigens, without using the original antigen and breaking antitumour tolerance.
通过用抗CA 125单克隆抗体OC125免疫小鼠，我们试图通过激活独特型网络来诱导针对肿瘤相关抗原CA125的抗体。通过反复给予OC125抗体的F(ab')2片段免疫的小鼠产生了抗独特型抗体，模拟了OC125的原始靶抗原。在诱导出这些抗独特型抗体（Ab2β）后，检测到了小鼠IgG抗CA125（Ab3）反应。独特型级联反应的诱导提供了在不使用原始抗原和打破抗肿瘤耐受性的情况下针对肿瘤相关抗原进行免疫的可能性。
