Veneroni S, Daidone M G, Di Fronzo G, Cappelletti V, Amadori D, Riccobon A, Paradiso A, Correale M, Silvestrini R
Oncologia Sperimentale C, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy.
Breast Cancer Res Treat. 1993;26(1):7-13. doi: 10.1007/BF00682695.
The expression of cathepsin D was evaluated by immunohistochemistry on histologic sections from formalin-fixed samples in a series of 436 primary breast cancers. The fraction of cathepsin D-positive tumor cells was not related to tumor size or hormone receptor status, and only weakly related to proliferative activity, evaluated as the 3H-thymidine labeling index. Conversely, a higher fraction of positive cells was observed in node-positive than in node-negative tumors (p = 0.05). A matched comparison between immunohistochemical and immunoradiometric results on individual tumors was carried out on 100 cases and showed a significant association but with a correlation coefficient of 0.46. The agreement of results from the two assays was higher in ER- than in ER+ tumors, which sometimes showed an immunostaining limited to macrophages and normal epithelial cells. In situ evaluation has the main advantage of being specifically applicable to detection in tumor cells and allows the simultaneous determination of different biologic aspects for a more complete understanding of breast cancer biology.
通过免疫组织化学方法，对436例原发性乳腺癌福尔马林固定样本的组织切片进行组织蛋白酶D表达评估。组织蛋白酶D阳性肿瘤细胞的比例与肿瘤大小或激素受体状态无关，与增殖活性（以3H-胸腺嘧啶核苷标记指数评估）仅有微弱关联。相反，在淋巴结阳性肿瘤中观察到的阳性细胞比例高于淋巴结阴性肿瘤（p = 0.05）。对100例个体肿瘤进行免疫组织化学和免疫放射分析结果的匹配比较，结果显示二者存在显著关联，但相关系数为0.46。雌激素受体阴性肿瘤中两种检测方法结果的一致性高于雌激素受体阳性肿瘤，后者有时免疫染色仅限于巨噬细胞和正常上皮细胞。原位评估的主要优点是特别适用于肿瘤细胞检测，并能够同时确定不同生物学方面的情况，以便更全面地了解乳腺癌生物学特性。
