James R, Dean D O, Debbage J
Molecular/Microbiology Sector, School of Biological Sciences, University of East Anglia, Norwich, Norfolk, England.
DNA Seq. 1993;3(5):327-32. doi: 10.3109/10425179309020832.
The nucleotide sequence of 3.17 kb of DNA upstream of the dnaK gene of E. coli was determined. Analysis of the sequence indicated the presence of five open reading frames (ORFs), two of which are coded on one strand and three on the other. ORFs 3 and 4 appear to constitute an operon. One of the ORFs (htgA) is preceded by a -10 promoter sequence which is identical to that of the dnaK sigma 32 P1 promoter, and thus suggests that htgA could be a heat-shock gene. ORF4 and htgA overlap each other on opposite strands of DNA, this would offer some interesting regulatory possibilities.
测定了大肠杆菌dnaK基因上游3.17 kb DNA的核苷酸序列。序列分析表明存在五个开放阅读框（ORF），其中两个在一条链上编码，另外三个在另一条链上编码。ORF3和ORF4似乎构成一个操纵子。其中一个开放阅读框（htgA）之前有一个-10启动子序列，该序列与dnaK σ32 P1启动子的序列相同，因此表明htgA可能是一个热休克基因。ORF4和htgA在DNA的相反链上相互重叠，这将提供一些有趣的调控可能性。
