丁香假单胞菌丁香致病变种61的hrpJ和hrpI基因的特性：HrpI与蛋白质转运相关蛋白超家族的同源性
Characterization of the Pseudomonas syringae pv. syringae 61 hrpJ and hrpI genes: homology of HrpI to a superfamily of proteins associated with protein translocation.
作者信息
Huang H C, Xiao Y, Lin R H, Lu Y, Hutcheson S W, Collmer A
机构信息
Agricultural Biotechnology Laboratories, National Chung-Hsing University, Taichung, Taiwan, Republic of China.
出版信息
Mol Plant Microbe Interact. 1993 Jul-Aug;6(4):515-20. doi: 10.1094/mpmi-6-515.
The Pseudomonas syringae pv. syringae 61 hrpJ and hrpI genes were sequenced and found to encode predicted proteins of 37,710 Da and 76,490 Da, respectively. The products of these genes were confirmed by T7 polymerase-dependent expression and sodium dodecyl sulfate-polyacrylamide gel analysis. HrpI belongs to a superfamily of proteins represented by Yersinia pestis LcrD.
对丁香假单胞菌丁香致病变种61的hrpJ和hrpI基因进行了测序，发现它们分别编码预测分子量为37710道尔顿和76490道尔顿的蛋白质。通过T7聚合酶依赖性表达和十二烷基硫酸钠-聚丙烯酰胺凝胶分析证实了这些基因的产物。HrpI属于以鼠疫耶尔森菌LcrD为代表的蛋白质超家族。
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