Jeffreys A J, Monckton D G, Tamaki K, Neil D L, Armour J A, MacLeod A, Collick A, Allen M, Jobling M
Department of Genetics, University of Leicester, England.
EXS. 1993;67:125-39. doi: 10.1007/978-3-0348-8583-6_12.
Most DNA typing systems assay allele length variation at tandemly repeated loci such as minisatellites and microsatellites. Allele length measurements are approximate, which impedes the use of such loci in forensic analysis and in studies of allelic variability at hypervariable loci. We now review progress in the development of alternative DNA typing systems based on allelic variation in the interspersion patterns of variant repeat units along minisatellite alleles. Minisatellite variant repeat mapping by PCR (MVR-PCR) not only provides a powerful new digital approach to DNA typing, but also for the first time allows investigation of the true level of allelic variability at minisatellite loci and of the mutational mechanisms that generate ultravariability.
大多数DNA分型系统检测串联重复位点（如小卫星和微卫星）处的等位基因长度变异。等位基因长度测量是近似值，这妨碍了此类位点在法医分析以及高变位点等位基因变异性研究中的应用。我们现在回顾基于小卫星等位基因上变异重复单元散布模式中的等位基因变异而开发的替代DNA分型系统的进展。通过聚合酶链反应进行小卫星变异重复图谱分析（MVR-PCR）不仅为DNA分型提供了一种强大的新数字方法，而且首次使得研究小卫星位点上等位基因变异的真实水平以及产生超变异性的突变机制成为可能。
