Nürnberg P, Berard J D, Bercovitch F, Epplen J T, Schmidtke J, Krawczak M
Institute für Medizinische Genetik, Medizinische Fakultät (Charité), Humboldt-Universität, Berlin, Germany.
EXS. 1993;67:445-51. doi: 10.1007/978-3-0348-8583-6_45.
Multilocus DNA fingerprinting with oligonucleotide probes (GTG)5, (GATA)4, and (CA)8 was applied in order to determine paternity in one birth cohort (15 infants) of social group (S) from the free-ranging colony of rhesus macaques (Macaca mulatta) on Cayo Santiago. While sires could be identified in 11 cases, all males tested (N = 19) could be excluded from paternity for the remaining four infants. Data revealed marked discrepancies between actual paternity and paternity as inferred from the observation of copulation behavior. Thus, a dominant social rank does not appear to be strongly associated with reproductive success. Furthermore, alternative reproductive strategies were found to yield comparable net benefits in reproduction. A second group of animals (M) was translocated from Cayo Santiago to the Sabana Seca Field Station in 1984. They have continuously resided together in a large outdoor enclosure since then. Here paternity assessment was seriously impeded by a reduced number of discriminating bands, i.e. offspring bands which were unequivocally derived from the sires. This was initially held to be indicative of a smaller degree of heterozygosity in Group M, and was attributed to inbreeding due to a lack of male immigration or extra-group fertilizations. However, a comparison of the DNA fingerprint patterns obtained in Group S and Group M lends only partial support to this idea.
为了确定圣地亚哥岛自由放养的恒河猴（猕猴属）社会群体（S）中一个出生队列（15只幼猴）的父系关系，应用了使用寡核苷酸探针（GTG）5、（GATA）4和（CA）8的多位点DNA指纹分析技术。虽然在11例中可以确定父系，但对其余4只幼猴进行检测的所有雄性（N = 19）都可以排除父系关系。数据显示实际父系关系与根据交配行为观察推断的父系关系之间存在明显差异。因此，占主导地位的社会等级似乎与繁殖成功没有强烈关联。此外，发现替代生殖策略在繁殖中产生了相当的净收益。1984年，第二组动物（M）从圣地亚哥岛转移到萨巴纳塞卡野外站。从那时起，它们一直共同生活在一个大型户外围栏中。在这里，父系评估受到区分条带数量减少的严重阻碍，即明确来自父系的后代条带。这最初被认为表明M组杂合度较低，并归因于缺乏雄性迁入或群体外受精导致的近亲繁殖。然而，对S组和M组获得的DNA指纹图谱进行比较，仅部分支持了这一观点。
