将蛋白质中的半胱氨酸作为还原水解产物中的半胱氨酸进行测定。
Determination of halfcystine in proteins as cysteine from reducing hydrolyzates.
作者信息
Sottrup-Jensen L
机构信息
Department of Molecular Biology, University of Aarhus, Denmark.
出版信息
Biochem Mol Biol Int. 1993 Jul;30(4):789-94.
PMID:8401334
Abstract
The separation of cysteine from proline using a variant of the classical cation exchange system for amino acid analysis is described. From reducing hydrolyzates (6 M HCl/0.1% phenol/5% thioglycollic acid, 18 h, 110 degrees C) the halfcystine content of proteins can be accurately determined as cysteine. The system is useful for routine determination of the composition of proteins from a single type of hydrolyzate.
摘要
本文描述了使用经典阳离子交换系统的一种变体进行氨基酸分析，以分离半胱氨酸和脯氨酸的方法。通过还原水解产物（6M盐酸/0.1%苯酚/5%巯基乙酸，18小时，110℃），蛋白质中的半胱氨酸含量可以准确地测定为半胱氨酸。该系统可用于从单一类型的水解产物中常规测定蛋白质的组成。
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