半胱氨酸：蛋白质和肽的巯基乙烷磺酸或盐酸水解产物氨基酸分析中潜在的误差来源。

Cysteine: a potential source of error in amino acid analysis of mercaptoethane sulfonic or hydrochloric acid hydrolysates of proteins and peptides.

作者信息

Gardner M L

出版信息

Anal Biochem. 1984 Sep;141(2):429-31. doi: 10.1016/0003-2697(84)90066-6.

Abstract

Hydrolysis of proteins and peptides with mercaptoethane sulfonic acid is liable to produce overestimation of the proline content owing to the production of ninhydrin-positive material (probably cysteine) which coelutes with proline on many ion-exchange analytical systems. A similar error occurs with HCl hydrolysis (especially in the presence of mercaptoethanol or thioglycollic acid) if care is not taken to oxidize cysteine during reconstitution of the hydrolysate before amino acid analysis.

摘要

用巯基乙烷磺酸水解蛋白质和肽时，由于产生茚三酮阳性物质（可能是半胱氨酸），在许多离子交换分析系统上与脯氨酸共洗脱，容易导致脯氨酸含量的高估。如果在氨基酸分析之前水解产物复溶过程中不注意氧化半胱氨酸，用盐酸水解（特别是在存在巯基乙醇或巯基乙酸的情况下）也会出现类似的误差。