Dewald G W, Schad C R, Christensen E R, Law M E, Zinsmeister A R, Stalboerger P G, Jalal S M, Ash R C, Jenkins R B
Division of Laboratory Genetics, Mayo Clinic, Rochester, MN 55905.
Bone Marrow Transplant. 1993 Aug;12(2):149-54.
We investigated the efficacy of fluorescence-labelled chromosome probes (CEP-X/Y) for the X and Y chromosomes to study patients who have had opposite sex BMT. These probes hybridize to the centromere region of the X chromosome and nearly the entire long arm of the Y chromosome. These probes are direct-labelled and produce X and Y signals that can be simultaneously viewed and readily distinguished from each other by color and size after only five brief washes. We investigated BM specimens from 20 normal donors and 16 patients who had undergone an opposite sex BMT. We found no significant interinvestigator differences with respect to scoring XX or XY interphase cells. The 'normal range' for XX cells in males was up to 0.628% and for XY cells in females it was up to 0.299%. Each of the specimens from the patients who underwent BMT had a significant number of donor cells compared with normal range. We suggest that an economical, rapid and accurate cytogenetic test can be achieved by using these probes as an adjunct to conventional cytogenetics.
我们研究了用于X和Y染色体的荧光标记染色体探针（CEP-X/Y）在研究接受异性骨髓移植（BMT）患者中的有效性。这些探针与X染色体的着丝粒区域以及Y染色体几乎整个长臂杂交。这些探针是直接标记的，产生的X和Y信号在仅经过五次简短洗涤后，就可以通过颜色和大小同时观察并轻易区分。我们研究了来自20名正常供体和16名接受异性BMT患者的骨髓标本。在对XX或XY间期细胞进行评分方面，我们未发现研究者之间存在显著差异。男性中XX细胞的“正常范围”高达0.628%，女性中XY细胞的“正常范围”高达0.299%。与正常范围相比，接受BMT患者的每个标本中都有大量的供体细胞。我们建议，将这些探针作为传统细胞遗传学的辅助手段，可以实现经济、快速且准确的细胞遗传学检测。
