人眼中X和Y染色体的间期荧光原位杂交
Interphase fluorescence in situ hybridisation of the X and Y chromosomes in the human eye.
作者信息
Wollensak G, Perlman E J, Green W R
机构信息
Eye Pathology Laboratory, Wilmer Eye Institute, Johns Hopkins University Medical Institutions, Baltimore, MD, USA.
出版信息
Br J Ophthalmol. 2001 Oct;85(10):1244-7. doi: 10.1136/bjo.85.10.1244.
AIM
To determine the sex of individual cells in paraffin sections of the human eye by fluorescence in situ hybridisation (FISH) of the X and Y chromosomes.
METHODS
The authors developed a protocol for FISH of the X and Y chromosomes in paraffin sections of human eyes.
RESULTS
In all the specimens that had been fixed in 10% formalin and with a fixation time of up to 3 days sex determination of individual cells was achieved. The percentage of cells with clearly identifiable signals was up to 98% for corneal epithelium, keratocytes, corneal endothelium, trabecular meshwork, lens epithelium, retina, and optic nerve.
CONCLUSIONS
FISH allows the determination of the sex of single cells in paraffin sections of human eyes without destruction of the tissue structure. Its main application is the histological analysis of sex mismatched corneal, RPE, or neuroretinal transplants to distinguish host and donor cells.
目的
通过对X和Y染色体进行荧光原位杂交（FISH）来确定人眼石蜡切片中单个细胞的性别。
方法
作者制定了一种用于人眼石蜡切片中X和Y染色体FISH的方案。
结果
在所有用10%福尔马林固定且固定时间长达3天的标本中，实现了单个细胞的性别鉴定。对于角膜上皮细胞、角膜细胞、角膜内皮细胞、小梁网、晶状体上皮细胞、视网膜和视神经，具有清晰可辨信号的细胞百分比高达98%。
结论
FISH能够在不破坏组织结构的情况下确定人眼石蜡切片中单个细胞的性别。其主要应用是对性别不匹配的角膜、视网膜色素上皮（RPE）或神经视网膜移植进行组织学分析，以区分宿主细胞和供体细胞。
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