Matsuoka M, Endou K, Yagi K, Nakajima Y
Division of Microbiology, Hokkaido Institute of Pharmaceutical Sciences, Japan.
Biol Pharm Bull. 1993 Jul;16(7):711-3. doi: 10.1248/bpb.16.711.
Staphylococcus aureus TPR-27, a clinically isolated strain, showed constitutive resistance to some macrolide antibiotics (erythromycin, oleandomycin, spiramycin, and josamycin), but susceptibility to the other macrolide (tylosin, rokitamycin, and mycinamicin), lincosamide, and streptogramin type B antibiotics (PM-resistance). The PM-resistant strain TPR-27 has carried for visible plasmids. Attempts to eliminate the resistant determinant in terms of ethidium bromide (about 3 micrograms/ml) did not succeed, and every trial to transduce the PM-resistant determinant into rec- mutant ISP105 using phage 80L2 propagated on strain TPR-27 also failed at the frequency of less than 1.1 x 10(-10) transductants per plaque-forming unit. These results suggest that the PM-resistance determinant is localized in chromosomal DNA.
金黄色葡萄球菌TPR - 27是一株临床分离菌株，对某些大环内酯类抗生素（红霉素、竹桃霉素、螺旋霉素和交沙霉素）表现出组成型抗性，但对其他大环内酯类抗生素（泰乐菌素、罗他霉素和麦迪霉素）、林可酰胺类抗生素以及B型链阳菌素类抗生素敏感（表现为对大环内酯 - 林可酰胺 - 链阳菌素B的抗性）。对大环内酯 - 林可酰胺 - 链阳菌素B耐药的菌株TPR - 27携带了可见质粒。用溴化乙锭（约3微克/毫升）消除抗性决定簇的尝试未成功，并且每次尝试使用在菌株TPR - 27上繁殖的噬菌体80L2将对大环内酯 - 林可酰胺 - 链阳菌素B的抗性决定簇转导到rec - 突变体ISP105中也以低于每噬菌斑形成单位1.1×10⁻¹⁰转导子的频率失败。这些结果表明，对大环内酯 - 林可酰胺 - 链阳菌素B的抗性决定簇定位于染色体DNA中。
