Matsuoka M, Jánosi L, Endou K, Saitoh S, Hashimoto H, Nakajima Y
Division of Microbiology, Hokkaido Institute of Pharmaceutical Sciences, Japan.
Biol Pharm Bull. 1993 Dec;16(12):1288-90. doi: 10.1248/bpb.16.1288.
A plasmid, pEP2104 (23.9 kilobase pairs), from Staphylococcus aureus carries a gene that specifies inducible resistance to 14-membered (erythromycin, EM, and oleandomycin, OL) and 16-membered macrolide (mycinamicin I and II), but not to all of the latter, and to streptogramin type B antibiotics (partial macrolide- and streptogramin-B-antibiotic resistance: PMS-resistance) (L. Jánosi, E. Bán, Acta Microbiol. Acad. Sci. Hung., 29, 187 (1982) and Y. Nakajima et al., J. Pharmacobio-Dyn., 15, 319 (1992)). The induced cells of strain 8325(pEP2104) did not inactivate EM, OL, josamycin, rokitamycin or mikamycin B (MKM-B), and the cell-free extract of the strain did not inactivate EM or MKM-B, either. Ribosomes from the cells whose PMS-resistance was induced by EM were sensitive not only to EM or spiramycin, but also to MKM-B. A 63000-dalton protein increased to a great extent only in the cell membrane fractions of induced 8325(pEP2104), and may be involved in PMS-resistance.
来自金黄色葡萄球菌的质粒pEP2104（23.9千碱基对）携带一个基因，该基因赋予对14元大环内酯类抗生素（红霉素、EM和竹桃霉素、OL）和16元大环内酯类抗生素（麦迪霉素I和II，但并非对所有后者）以及链阳菌素B类抗生素的诱导型抗性（部分大环内酯类和链阳菌素B类抗生素抗性：PMS抗性）（L. 亚诺西、E. 班，《匈牙利科学院微生物学报》，29，187（1982年）以及Y. 中岛等人，《药物生物动力学杂志》，15，319（1992年））。8325(pEP2104)菌株的诱导细胞不会使EM、OL、交沙霉素、罗他霉素或米卡霉素B（MKM - B）失活，该菌株的无细胞提取物也不会使EM或MKM - B失活。由EM诱导出PMS抗性的细胞中的核糖体不仅对EM或螺旋霉素敏感，对MKM - B也敏感。一种63000道尔顿的蛋白质仅在诱导型8325(pEP2104)的细胞膜组分中大量增加，可能与PMS抗性有关。
