Château M T, Caravano R
INSERM U65, Département de Biologie-Santé, Université de Montpellier II, France.
FEMS Immunol Med Microbiol. 1993 Aug;7(2):111-8. doi: 10.1111/j.1574-695X.1993.tb00389.x.
The human histiocytic lymphoma cell line U937 can be induced to differentiate along the monocyte/macrophage pathway by either phorbol myristate acetate (PMA) or by the combination of retinoic acid (RA) and 1,25-dihydroxyvitamin D3 (VD). U937 cells treated with either PMA or RA/VD were able to phagocytose Salmonella typhimurium in the presence of non-immune human serum. However, only cells differentiated by RA/VD were capable of developing an oxidative metabolic burst in response to infection. Since the oxidative burst is considered to be a potent antimicrobial mechanism, we investigated its effect on S. typhimurium. The oxidative burst failed to affect either the viability or the multiplication of S. typhimurium suggesting that it plays only a minor role in the host defence against S. typhimurium.
人组织细胞淋巴瘤细胞系U937可通过佛波醇肉豆蔻酸酯乙酸盐（PMA）或视黄酸（RA）与1,25 - 二羟基维生素D3（VD）的组合诱导沿单核细胞/巨噬细胞途径分化。用PMA或RA/VD处理的U937细胞能够在非免疫人血清存在的情况下吞噬鼠伤寒沙门氏菌。然而，只有通过RA/VD分化的细胞能够在感染时产生氧化代谢爆发。由于氧化爆发被认为是一种强大的抗菌机制，我们研究了其对鼠伤寒沙门氏菌的影响。氧化爆发未能影响鼠伤寒沙门氏菌的活力或增殖，这表明它在宿主抵御鼠伤寒沙门氏菌的防御中仅起次要作用。
