De Azevedo N L, De Souza W
Laboratorio de Ultraestrutura Celular Hertha Meyer, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.
J Eukaryot Microbiol. 1993 Sep-Oct;40(5):636-42. doi: 10.1111/j.1550-7408.1993.tb06121.x.
The fine structure of normal and antibody-coated Tritrichomonas foetus cells and their interaction with rat peritoneal neutrophils was studied. Peritoneal neutrophils were obtained by glycogen stimulation. The neutrophils readily associated with and killed the parasites, which were subsequently ingested. The process involved activation of the respiratory burst, as demonstrated by the use of cytochemical methods. Images were obtained indicating that binding of parasites to the neutrophil surface triggers an exocytic response with release of oxygen-derived products. Cytochemical localization of acid phosphatase and peroxidase activities showed that leukocyte granules fused with the parasite-containing phagocytic vacuoles. We also showed the cytochemical localization of alkaline phosphatase in the parasite-neutrophil interaction.
研究了正常和抗体包被的胎儿三毛滴虫细胞的精细结构及其与大鼠腹腔中性粒细胞的相互作用。通过糖原刺激获得腹腔中性粒细胞。中性粒细胞很容易与寄生虫结合并将其杀死，随后寄生虫被吞噬。通过细胞化学方法证明，该过程涉及呼吸爆发的激活。获得的图像表明，寄生虫与中性粒细胞表面的结合引发了胞吐反应，并释放出氧衍生产物。酸性磷酸酶和过氧化物酶活性的细胞化学定位表明，白细胞颗粒与含有寄生虫的吞噬泡融合。我们还展示了碱性磷酸酶在寄生虫与中性粒细胞相互作用中的细胞化学定位。
