de Azevedo N L, de Souza W
Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Brazil.
J Submicrosc Cytol Pathol. 1996 Apr;28(2):243-9.
The fine structure of the process of interaction between uncoated and antibody-coated Tritrichomonas foetus with rat peritoneal eosinophils was studied. Eosinophils were purified by discontinuous Metrizamide gradient. Agglutination and immunofluorescence microscopy showed that T.foetus grown in a medium supplemented with different kinds of serum, antibodies present in it opsonize the parasites. Parasites incubated in the presence of anti-T.foetus rabbit serum attach to and are ingested by eosinophils. No such attachment was observed with trypsin-treated parasites. Attachment of antibody-coated parasites to the eosinophils induced their degranulation with the release of granule contents onto parasite surface causing its destruction. Hyperimmune serum was shown to be required for both attachment and ingestion of the parasites. The cytochemical localization of basic proteins and peroxidase showed that leucocyte granules fused with parasite-containing phagocytic vacuoles. Images were obtained suggesting that the binding of the parasites to the eosinophil surface triggers an exocytic process with release of the granule content onto the parasite surface.
研究了未包被抗体和包被抗体的胎儿三毛滴虫与大鼠腹腔嗜酸性粒细胞相互作用过程的精细结构。嗜酸性粒细胞通过不连续的甲泛葡胺梯度进行纯化。凝集和免疫荧光显微镜检查表明，在添加不同种类血清的培养基中生长的胎儿三毛滴虫，其中存在的抗体可调理这些寄生虫。在抗胎儿三毛滴虫兔血清存在下孵育的寄生虫会附着于嗜酸性粒细胞并被其吞噬。经胰蛋白酶处理的寄生虫未观察到这种附着现象。包被抗体的寄生虫附着于嗜酸性粒细胞会诱导其脱颗粒，颗粒内容物释放到寄生虫表面导致其破坏。结果表明，超免疫血清对于寄生虫的附着和吞噬均是必需的。碱性蛋白和过氧化物酶的细胞化学定位显示，白细胞颗粒与含有寄生虫的吞噬泡融合。获得的图像表明，寄生虫与嗜酸性粒细胞表面的结合触发了一个胞吐过程，颗粒内容物释放到寄生虫表面。
