Robak J, Gryglewski R J
Department of Pharmacology, N. Copernicus Medical Academy, Kraków, Poland.
Pol J Pharmacol. 1993 Jan-Feb;45(1):51-8.
Three classical direct nitric oxide (NO) donors, 3-morpholine-sydnonimine (SIN-1), S-nitroso-N-acetyl-d,l-penicillamine (SNAP) and sodium nitroprusside (NaNP) as well as two indirect NO donors, molsidomine (MSL) and glyceryl trinitrate (GTN) were studied for their potencies to generate O2-, to scavenge O2-, to consume molecular oxygen and to inhibit lipid oxidation. Out of five NO donors only those which were spontaneous releasers of NO at physiological pH were also scavengers of O2- which has been generated by xanthine:xanthine oxidase system (SIN-1 IC50 19 microM, SNAP IC50 416 microM) and inhibitors of the Fe3+ and ascorbate stimulated oxidation of rat liver lipids (SIN-1 IC50 76 microM, SNAP IC50 12 microM). Only SIN-1 at high concentrations of 300-5000 microM generated O2- as detected by a SOD inhibitable reduction of nitroblue tetrazolium. None of the in vitro studied activities were exerted by NaNP, MLS and GTN.
研究了三种经典的直接一氧化氮（NO）供体，即3-吗啉代西多胺（SIN-1）、S-亚硝基-N-乙酰-d，l-青霉胺（SNAP）和硝普钠（NaNP），以及两种间接NO供体，莫索尼定（MSL）和甘油三硝酸酯（GTN），考察它们产生超氧阴离子（O2-）、清除O2-、消耗分子氧以及抑制脂质氧化的能力。在这五种NO供体中，只有那些在生理pH下能自发释放NO的物质才同时也是黄嘌呤：黄嘌呤氧化酶系统产生的O2-的清除剂（SIN-1的IC50为19 microM，SNAP的IC50为416 microM）以及Fe3+和抗坏血酸刺激的大鼠肝脏脂质氧化的抑制剂（SIN-1的IC50为76 microM，SNAP的IC50为12 microM）。只有高浓度（300 - 5000 microM）的SIN-1能产生O2-，这可通过超氧化物歧化酶（SOD）抑制的硝基蓝四唑还原反应检测到。NaNP、MSL和GTN在体外研究中均未表现出任何活性。
